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PROCEDURE TO MOUNT A TEMPORARY SLIDE OF MITOSIS OF ONION ROOT TIP Aim : To prepare a temporary slide of mitosis stages using onion root tip.

Materials required: 1.onion root tip with pointed end 2.distilled water 3. embryo cub (2) 4. slide (1) 5.cover slip(1) 6.blotting paper 7.needle 8. acetocarmine solution for staining 9.1 N Hydrochloric acid 10. microscope for observing slide and 11.spirit lamp.

Procedure to be followed: 1. Take a embryo cup filled with 1 N hydrochloric acid. 2. select a onion root tip with pointed end and put it into 1 N hydrochloric acid for 15 minutes for loosening of the tissue. 3. wash the onion root tip with distilled water. 4. Take another embryo cup filled with acetocarmine solution and put the root tip in it for 25 minutes. 5. After 25 minutes take out the onion root tip from embryo cup and transfer it on the slide. 6. put some acetocarmine solution on slide. 7. heat the slide gently over the flame for few seconds. 8. Put the cover slip over the material taking care that air bubbles are not formed during this.

9. Wrap the slide with blotting paper or filter paper. 10. Tap the slide using the blunt end of pencil or pen for evenly spreading of material. 11. Remove the blotting paper and observe under the microscope.

Observation: Different stages of mitosis cell divisions are observed i.e Pro phase, Anaphase , Meta phase and Telophase. https://s-media-cache-ak0.pinimg.com/736x/96/3a/0e/963a0e326590f23fda07a6f6ec1f6b39.jpg http://www1.doc-stock.com/doStpremid/000202000/202213.jpg