User:Dekhang migmar/Sandbox

[[File:Preparation of slides to study different stages of mitosis . Aim : to study the different stages of mitosis of the root apical cell. Materials required : root apex, HCl , ferric acetate or rusted nail,  slide ,coverslip , blade , forcep , brush , needle and  petridish Procedure : Take 2 to 3 small  pieces of freshly grown root apex and put it in 1N of HCl in the petridish.keep it for 10mins. Then take 9 drops of acetocaramine, 1 drop of 1N HCl and use a rusted needle to stir it well and put the sample in it and keep it aside for 15 mins. Then warm it very slightly and keep it aside for 3mins . Take the sample and cut the apical side of the root which it pointed and then add 2 drops of acetocarmine and put coverslip and cover it with blotting paper and press it with the help of thumb and remove the blotting paper without disturbing the coverslip so that overlapping of cells should be avoided. Observe the slide under microscope.